Plasmid_Backbone

Part:BBa_M36095:Design

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-23)


S. elongatus cyanobacteria sensor test rig


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 2489
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2505
    Illegal BamHI site found at 2515
    Illegal BamHI site found at 5060
    Illegal XhoI site found at 3234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
    Illegal NgoMIV site found at 5086
    Illegal NgoMIV site found at 5458
    Illegal NgoMIV site found at 5618
    Illegal NgoMIV site found at 5972
    Illegal AgeI site found at 1913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2776
    Illegal BsaI.rc site found at 8122
    Illegal SapI site found at 7039


Design Notes

Redesigned by the BIOE44 teaching staff from pNS2-YFP.

Source

Source plasmid is pNS2-YFP. pNS2-YFP is a plasmid made by Rachel Hood from Dr. Dave Savage's lab.

References